spliced leader การใช้

• Some species contain only one spliced leader sequence found on all mRNAs.
• Spliced leaders have been seen in trypanosomatids, Euglena, flatworms, Caenorhabditis.
• The acquisition of a spliced leader from an SL RNA is an inter-molecular reaction that precisely joins exons derived from separately transcribed RNAs.
• Targets include ribosomal and spliceosomal RNAs as well as the Trypanosoma spliced leader RNA ( SL RNA ) as possibly other, still unknown cellular RNAs.
• These have names like "'CEOP5460 "'and are manually curated using evidence from the SL2 trans-spliced leader sequence sites.
• Spliced leader sequences are short sequences of non coding RNA, not found within a gene itself, that are attached to the 5 end of all, or a portion of, mRNAs transcribed in an organism.
• The " L . major " and " L . infantum " genomes contain only about 50 copies of inactive degenerated " Ingi " / L1Tc-related elements ( DIREs ), while " L . braziliensis " also contains several telomere-associated transposable elements and spliced leader-associated retroelements.
• However, other data types ( e . g . protein alignments, " ab initio " prediction programs, trans-splice leader sites, poly-A signals and addition sites, SAGE and TEC-RED transcript tags, mass-spectroscopic peptides, and conserved protein domains ) are useful in refining the structures, especially where expression is low and so transcripts are not sufficiently available.